کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2147683 | 1548421 | 2006 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Bystander-induced differentiation: A major response to targeted irradiation of a urothelial explant model
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Bystander-induced differentiation: A major response to targeted irradiation of a urothelial explant model Bystander-induced differentiation: A major response to targeted irradiation of a urothelial explant model](/preview/png/2147683.png)
چکیده انگلیسی
A ureter primary explant technique, using porcine tissue sections was developed to study bystander effects under in vivo like conditions where dividing and differentiated cells are present. Targeted irradiations of ureter tissue fragments were performed with the Gray Cancer Institute charged particle microbeam at a single location (2 μm precision) with 10 3He2+ particles (5 MeV; LET 70 keV/μm). After irradiation the ureter tissue section was incubated for 7 days allowing explant outgrowth to be formed. Differentiation was estimated using antibodies to Uroplakin III, a specific marker of terminal urothelial differentiation. Even although only a single region of the tissue section was targeted, thousands of additional cells were found to undergo bystander-induced differentiation in the explant outgrowth. This resulted in an overall increase in the fraction of differentiated cells from 63.5 ± 5.4% to 76.6 ± 5.6%. These changes are much greater than that observed for the induction of damage in this model. One interpretation of these results is that in the tissue environment, differentiation is a much more significant response to targeted irradiation and potentially a protective mechanism.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis - Volume 597, Issues 1â2, 11 May 2006, Pages 43-49
Journal: Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis - Volume 597, Issues 1â2, 11 May 2006, Pages 43-49
نویسندگان
Oleg V. Belyakov, Melvyn Folkard, Carmel Mothersill, Kevin M. Prise, Barry D. Michael,