کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2149252 | 1089609 | 2007 | 8 صفحه PDF | دانلود رایگان |
Inflammation is known to be an important underlying condition in the development of a variety of diseases. To investigate whether blood lead induces inflammatory reactions in non-occupationally exposed adults and the effects of genetic susceptibility associated with GSTM1 and TNF-α gene polymorphisms on this inflammatory response, we measured blood lead levels in 300 healthy university students. Total serum TNF-α and IL-6 levels and WBC counts were determined to evaluate the inflammatory response. Allelic loss of GSTM1 and the TNF-α–308 G > A polymorphism were determined by PCR and RFLP. Positive relations between blood lead and three inflammation biomarkers were shown in male subjects with blood lead ≥2.51 μg/dl (median value) (TNF-α, p = 0.015; IL-6, p = 0.082; and WBC, p = 0.044). However, subgroup analysis by genotype showed an effect of blood lead on the three biomarkers only in individuals with the GSTM1 null (TNF-α, p = 0.020; IL-6, p = 0.096; and WBC, p = 0.017) or TNF-α GG (TNF-α, p = 0.017; IL-6, p = 0.088; and WBC, p = 0.095) genotype, and not in individuals with GSTM1 present (all three inflammatory biomarkers, p > 0.1) or the TNF-α GA or AA (all three biomarkers, p > 0.1) genotype. These results suggest that blood lead affects the inflammatory response and that GSTM1 and TNF-α gene polymorphisms are genetic factors associated with lead-induced inflammatory response.
Journal: Mutation Research/Genetic Toxicology and Environmental Mutagenesis - Volume 629, Issue 1, 20 April 2007, Pages 32–39