کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2153933 1090212 2011 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Preparation and biological evaluation of 177Lu conjugated PR81 for radioimmunotherapy of breast cancer
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی تحقیقات سرطان
پیش نمایش صفحه اول مقاله
Preparation and biological evaluation of 177Lu conjugated PR81 for radioimmunotherapy of breast cancer
چکیده انگلیسی

AimPR81 is a monoclonal antibody that binds with high affinity to MUC1 antigen that is over expressed in 80% of breast cancers. In this study, we developed a method for indirect labeling of PR81 with lutetium-177 and performed all preclinical qualifications in production of a biologic agent for radioimmunotherapy of breast cancer.Materials and MethodsThe radiochemical purity and in vitro stability of 177Lu labeled PR81 was determined by instant thin layer chromatography. The immunoreactivity and cell toxicity of the complex were tested on MCF7 cell line. The biodistribution and scintigraphy studies were performed in BALB/c mice with breast tumor.ResultsThe radiochemical purity was 91.2±3.8% after 2 h. The in vitro stabilities in phosphate buffer and human blood serum were 83.1±3.4% and 76.2±3.6% at 96 h, respectively. The immunoreactivity of the complex was 83.4±2.4%. The cell toxicity study showed that the complex inhibited 85.2±3.4% growth of MCF7 cells at a concentration of 2500 ng/ml after 96 h. The biodistribution and scintigraphy studies showed the accumulation of the complex at the site of tumors with high sensitivity and specificity.ConclusionThe results showed that one may consider 177Lu-DOTA–PR81 as a potential radiopharmaceutical for therapy of human breast cancer, which needs further investigations.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Nuclear Medicine and Biology - Volume 38, Issue 6, August 2011, Pages 849–855
نویسندگان
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