کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2154811 1090253 2006 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Comparison of [18F]FDOPA, [18F]FMT and [18F]FECNT for imaging dopaminergic neurotransmission in mice
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی تحقیقات سرطان
پیش نمایش صفحه اول مقاله
Comparison of [18F]FDOPA, [18F]FMT and [18F]FECNT for imaging dopaminergic neurotransmission in mice
چکیده انگلیسی

IntroductionThe clinically established positron emission tomography (PET) tracers 6-[18F]-fluoro-l-DOPA ([18F]FDOPA), 6-[18F]-fluoro-l-m-tyrosine ([18F]FMT) and 2β-carbomethoxy-3β-(4-chlorophenyl)-8-(2-[18F]-fluoroethyl)-nortropane ([18F]FECNT) serve as markers of presynaptic integrity of dopaminergic nerve terminals in humans. This study describes our efforts to adopt the methodology of human Parkinson's disease (PD) PET studies to mice.MethodsThe PET imaging characteristics of [18F]FDOPA, [18F]FMT and [18F]FECNT were analyzed in healthy C57BL/6 mice using the dedicated small-animal PET tomograph quad-HIDAC. Furthermore, [18F]FECNT was tested in the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) mouse model of PD.Results[18F]FDOPA and [18F]FMT failed to clearly visualize the mouse striatum, whereas PET experiments using [18F]FECNT proved that the employed methodology is capable of delineating the striatum in mice with exquisite resolution. Moreover, [18F]FECNT PET imaging of healthy and MPTP-lesioned mice demonstrated that the detection and quantification of striatal degeneration in lesioned mice can be accomplished.ConclusionsThis study shows the feasibility of using [18F]FECNT PET to analyze noninvasively the striatal degeneration in the MPTP mouse model of PD. This methodology can be therefore considered as a viable complement to established in vivo microdialysis and postmortem techniques.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Nuclear Medicine and Biology - Volume 33, Issue 5, July 2006, Pages 607–614
نویسندگان
, , , , , , ,