کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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217700 | 1426421 | 2007 | 7 صفحه PDF | دانلود رایگان |
One of the difficulties encountered in the control of bioprocesses is the lack of reliable on-line sensors for key state variables. On-line measurements of the recombinant biomass and intracellular protein are the key requirements to achieving an effective process operation. This paper investigates the suitability of using on-line estimation to predict concentrations of biomass and protein for recombinant Escherichia coli cultivated in batch and fed-batch modes. Various pairs of on-line accessible measurements of glucose, lactate, and acetate are fed back into the on-line estimator to train the tuning parameters in order to predict concentrations of biomass and intracellular protein. Such predictions are consistent with experimental data using off-line measurements.
Journal: Journal of the Chinese Institute of Chemical Engineers - Volume 38, Issues 3–4, May–July 2007, Pages 197–203