کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2182755 | 1095514 | 2016 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Alt a 15 is a new cross-reactive minor allergen of Alternaria alternata
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
CCDPVDFAlternaria alternataSDAPSpecific IgE - IgE اختصاصیrecombinant allergen - آلرژن نوترکیبFungal allergy - آلرژی قارچیcross-reactive carbohydrate determinants - تعیین کننده های کربوهیدرات متقاطع واکنش پذیرrapid amplification of cDNA ends - تقویت سریع cDNA به پایان می رسدpolyvinylidene difluoride - دی فلوئورید پلی وینیلیدینSerine protease - سرین پروتئازRace - مسابقهCross-reactivity - واکنش پذیری متقابلcDNA cloning - کلونینگ cDNAsIgE - گفتن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Alternaria alternata is one of the most common saprophytes worldwide that is clinically and epidemiologically associated with severe asthma. Therefore, the identification and characterization of all A. alternata allergens are of major clinical importance. This study describes a new cross-reactive A. alternata allergen that was officially named Alt a 15 by the official Allergen Nomenclature Subcommittee. The complete coding region for Alt a 15 was amplified using 5Ⲡand 3Ⲡrapid amplification of cDNA ends and PCR. The recombinant protein was produced in Escherichia coli as a 65-kDa fusion protein, and the protein sequence exhibits high homology with several important fungal allergens. Immunoblotting analyses revealed that IgE antibodies from A. alternata-sensitized patients (n = 59) bound to rAlt a 15 with a prevalence of 10.2%. All patients who presented sIgE to rAlt a 15 were apparently poly-sensitized to A. alternata and C. lunata. The extensive cross-reactivity between A. alternata and C. lunata serine proteases was confirmed using immunoblotting inhibition assays. Overall, Alt a 15 is an important new cross-reactive allergen of A. alternata that explains some allergies to A. alternata without Alt a 1 sensitization and initial diagnostic errors for allergies to Alternaria. This molecule may improve the accuracy of the diagnosis, the understanding, and the management of IgE-mediated fungal diseases.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Immunobiology - Volume 221, Issue 2, February 2016, Pages 153-160
Journal: Immunobiology - Volume 221, Issue 2, February 2016, Pages 153-160
نویسندگان
M.F. Gabriel, I. Postigo, A. Gutiérrez-RodrÃguez, E. Suñén, J.A. Guisantes, J. Fernández, C.T. Tomaz, J. MartÃnez,