کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2183165 | 1095551 | 2014 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Human macrophage polarization in vitro: Maturation and activation methods compared
ترجمه فارسی عنوان
پلاریزاسیون ماکروفاژ انسانی در آزمایشگاهی: روشهای بلوغ و فعال سازی در مقایسه با
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کلمات کلیدی
MFINHSGM-CSFM-CSFWound Healing - التیام زخمinterleukin - اینترلوکینnormal human serum - سرم طبیعی انسانMacrophage activation - فعال سازی ماکروفاژFibrosis - فیبروز یا فساد الیافlipopolysaccharide - لیپوپلی ساکاریدmacrophage colony-stimulating factor - ماکروفاژ عامل کلونی تحریک کنندهalternatively activated macrophage - ماکروفاژ فعال متناوبgranulocyte macrophage colony-stimulating factor - ماکروفاژ گرانولوسیت عامل کلونی تحریک کنندهMultiple sclerosis - مولتیپل اسکلروزیس(ام اس)mean fluorescence intensity - میانگین شدت فلورسانسMannose receptor - گیرنده مانوز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
چکیده انگلیسی
Macrophages form a heterogeneous cell population displaying multiple functions, and can be polarized into pro- (M1) or anti-inflammatory (M2) macrophages, by environmental factors. Their activation status reflects a beneficial or detrimental role in various diseases. Currently several in vitro maturation and activation protocols are used to induce an M1 or M2 phenotype. Here, the impact of different maturation factors (NHS, M-CSF, or GM-CSF) and activation methods (IFN-γ/LPS, IL-4, dexamethason, IL-10) on the macrophage phenotype was determined. Regarding macrophage morphology, pro-inflammatory (M1) activation stimulated cell elongation, and anti-inflammatory (M2) activation induced a circular appearance. Activation with pro-inflammatory mediators led to increased CD40 and CD64 expression, whereas activation with anti-inflammatory factors resulted in increased levels of MR and CD163. Production of pro-inflammatory cytokines was induced by activation with IFN-γ/LPS, and TGF-β production was enhanced by the maturation factors M-CSF and GM-CSF. Our data demonstrate that macrophage marker expression and cytokine production in vitro is highly dependent on both maturation and activation methods. In vivo macrophage activation is far more complex, since a plethora of stimuli are present. Hence, defining the macrophage activation status ex vivo on a limited number of markers could be indecisive. From this study we conclude that maturation with M-CSF or GM-CSF induces a moderate anti- or pro-inflammatory state respectively, compared to maturation with NHS. CD40 and CD64 are the most distinctive makers for human M1 and CD163 and MR for M2 macrophage activation and therefore can be helpful in determining the activation status of human macrophages ex vivo.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Immunobiology - Volume 219, Issue 9, September 2014, Pages 695-703
Journal: Immunobiology - Volume 219, Issue 9, September 2014, Pages 695-703
نویسندگان
Daphne Y.S. Vogel, Judith E. Glim, Andrea W.D. Stavenuiter, Marjolein Breur, Priscilla Heijnen, Sandra Amor, Christine D. Dijkstra, Robert H.J. Beelen,