کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2183433 | 1095566 | 2010 | 9 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Distribution of macrophages and T cells in syngrafts and allografts after experimental rat lung transplantation Distribution of macrophages and T cells in syngrafts and allografts after experimental rat lung transplantation](/preview/png/2183433.png)
Macrophages and T cells have a pivotal role in orchestrating the acute lung allograft rejection response. We investigated the spatial and temporal distribution of these immune cells and the synthesis patterns of the Th1- and Th2-cytokine IL-12 and IL-10 during the early course after transplantation (Tx). Orthotopic single-lung Tx was performed in Lewis to Lewis (syngrafts) and Brown Norway/Lewis F1 hybrid to Lewis (allografts). Naïve lungs, syngrafts after 5 days and allografts after 3 and 5 days were analyzed for CD68+, CD163+ and CD3+ cells by immunohistochemistry and IL-12 and IL-10 were detected by immunofluorescence. CD68+ macrophages increased in number after allogeneic Tx compared to syngeneic Tx on day 5 (P<.001), CD163+ macrophages sequestrated early around veins (day 3) compared to the accumulation around arteries and bronchioles (P<.001) while CD3+ T cells were scarce. There was a predominance of IL-12 over IL-10 on day 5 after allogeneic Tx (P<.001). CD68+ macrophages were the most abundant cells during acute pulmonary rejection and CD163+ macrophages showed a characteristic distribution pattern over time around vessels and bronchioles. The up-regulation of IL-12 reflects an early response after allo-antigen exposure, indicating a strong impact of the initiation of the Th1 pathway at an early phase during acute lung rejection.
Journal: Immunobiology - Volume 215, Issue 3, March 2010, Pages 206–214