کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2184142 1095643 2007 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Ex vivo characterization of human thymic dendritic cell subsets
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
Ex vivo characterization of human thymic dendritic cell subsets
چکیده انگلیسی

Interactions between thymic dendritic cells (DC) and thymocytes are critical for proper development of T-cells. We identified human thymic DC populations on the basis of CD123, CD11c and CD14 expression. High levels of CD123 (IL-3R) and CD45RA defined the plasmacytoid DC (pDC) subset. Human thymic CD11c+ DC expressed CD45RO and myeloid-related markers (CD13, CD33 and CD11b). CD11c+ DC could be separated into two main subsets based on differential expression of CD14: CD11c+ CD14− and CD11c+ CD14+ cells. Spontaneous production of IL-10 and IFNγ without exogenous stimulation, was observed in the three DC subsets. Important phenotype modifications were observed in pDC cultures supplemented with IL-3. A down-regulation of CD123 and appearance of myeloid markers such as CD11b and CD11c on CD45RA+ cells was noticed within the first 48 h; at a later time there was a shift from CD45RA to CD45RO expression, as well as appearance of CD14 expression. CD11c+ cells emerging in pDC culture did not express high levels of HLA-DR, CD83 and co-stimulatory molecules. This suggests an in vitro evolution of human thymic pDC toward a myeloid phenotype found in the CD11c+ subset of thymic DC.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Immunobiology - Volume 212, Issue 3, 15 May 2007, Pages 167–177
نویسندگان
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