کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2203501 1100502 2016 15 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Quantitative approach to lectin-based glycoprofiling of thymic tissues in the control- and the dexamethasone-treated mice
ترجمه فارسی عنوان
رویکرد کمی به گلیکوپروفیفی کردن بافت لکتین در بافتهای سمی در موشهای کنترل شده و دگزامتازون
کلمات کلیدی
دگزامتازون، هیستوشیمی لکتین، تامیس گلیکوپروفایلینگ
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم کشاورزی و بیولوژیک (عمومی)
چکیده انگلیسی


• The effect of dexamethasone on thymic glycans was quantitatively evaluated.
• The glycoprofile of thymus changed upon the 24 h dexamethasone administration.
• Dexamethasone can affect the fate of thymocytes by modulating glycosylation pathways.

Dexamethasone (DEX) is the most commonly used synthetic glucocorticoid in treatment of various inflammatory conditions. Here we focused on evaluating the effect of DEX on apoptosis and glycan profile in the mouse thymic tissues. Histological examinations revealed that the DEX treatment cause severe alterations in thymus, such as disruption of thymic capsule, impaired epithelial cell-thymocyte contacts, cellular loss and increased apoptosis. The identification of thymic glycans in the control- and the DEX-treated mice was carried out by using a panel of five plant lectins, Maackia amurensis agglutinin (MAA), peanut agglutinin (PNA), Sambucus nigra agglutinin (SNA), Concanavalin A (ConA) and wheat germ agglutinin (WGA). Lectin histochemistry results showed that glycosylation pattern of thymus changes upon DEX treatment. For further detailed quantitative analyses of the binding intensities for each lectin, histochemical data were scored as high positive (HP), mild positive (MP) and low positive (LP) and differences among signaling densities were investigated. The staining patterns of thymic regions observed with lectin histochemistry suggest that DEX can affect the thymic glycan profile as well as thymocyte apoptosis. These results are consistent with the opinion that not only sialic acid, but also other sugar motifs may be responsible for thymocyte development.

Figure optionsDownload high-quality image (211 K)Download as PowerPoint slide

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Tissue and Cell - Volume 48, Issue 3, June 2016, Pages 168–182
نویسندگان
,