کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2204010 | 1551413 | 2008 | 9 صفحه PDF | دانلود رایگان |
We identified that nestin-EGFP-positive cells isolated by fluorescent-activated cell sorting (FACS) were downstream progenitor cells from colony-initiating pancreatic precursor cells. Under differentiation condition, these nestin-EGFP-positive cells could generate islet and neural cells. To investigate the conditions that allowed nestin-EGFP-positive progenitor cells (NPPCs) to efficaciously differentiate into insulin-producing cells in vitro, protocols were designed with glucagon-like peptide-1 (GLP-1) and the histone deacetylase inhibitor, sodium butyrate. We demonstrated that the combination of GLP-1 and sodium butyrate resulted in the increasing of levels of transcripts encoding pancreatic developmental factors and insulin. As a consequence, the amount of insulin-producing cells and insulin secretion were enhanced. These results indicated that NPPCs which were cultured in the presence of GLP-1 and sodium butyrate could specially differentiate into insulin-producing cells.
Journal: Tissue and Cell - Volume 40, Issue 6, December 2008, Pages 437–445