کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
227122 464815 2015 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Enzymatic synthesis of amentoflavone glycoside using recombinant oleandomycin glycosyltransferase
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی مهندسی شیمی (عمومی)
پیش نمایش صفحه اول مقاله
Enzymatic synthesis of amentoflavone glycoside using recombinant oleandomycin glycosyltransferase
چکیده انگلیسی

Glycosyltransferase (GT) can convert flavonoids, alkaloids, and terpenoids into glycosylated forms. An oleandomycin glycosyltransferase, “OleD” GT from Streptomyces antibioticus was exploited for the glycosylation of amentoflavone. The OleD was functionally expressed in Escherichia coli BL21 (DE3) and purified enzyme was employed as the biocatalyst for the glycosylation of amentoflavone via the transfer of glucose moiety from UDP (uridine diphosphate)-glucose. As a result, amentoflavone monoglycoside was evident in HPLC and LC–ESI-MS spectrometry with an exact mass of 701. In addition, the reaction conditions were optimized with the highest conversion up to 85% at 30 min.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Industrial and Engineering Chemistry - Volume 25, 25 May 2015, Pages 304–307
نویسندگان
, , , , ,