کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
227122 | 464815 | 2015 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Enzymatic synthesis of amentoflavone glycoside using recombinant oleandomycin glycosyltransferase
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موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
مهندسی شیمی (عمومی)
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چکیده انگلیسی
Glycosyltransferase (GT) can convert flavonoids, alkaloids, and terpenoids into glycosylated forms. An oleandomycin glycosyltransferase, “OleD” GT from Streptomyces antibioticus was exploited for the glycosylation of amentoflavone. The OleD was functionally expressed in Escherichia coli BL21 (DE3) and purified enzyme was employed as the biocatalyst for the glycosylation of amentoflavone via the transfer of glucose moiety from UDP (uridine diphosphate)-glucose. As a result, amentoflavone monoglycoside was evident in HPLC and LC–ESI-MS spectrometry with an exact mass of 701. In addition, the reaction conditions were optimized with the highest conversion up to 85% at 30 min.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Industrial and Engineering Chemistry - Volume 25, 25 May 2015, Pages 304–307
Journal: Journal of Industrial and Engineering Chemistry - Volume 25, 25 May 2015, Pages 304–307
نویسندگان
Amit Kumar Chaudhary, In Yeub Hwang, Yoon Ju Jo, Sung Hee Choi, Eun Yeol Lee,