کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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228423 | 464840 | 2012 | 6 صفحه PDF | دانلود رایگان |
In this study, Enzyme activities of krill were characterized before and after lipid extraction by supercritical carbon dioxide (SC-CO2) and organic solvent, n-hexane and acetone. Krill SC-CO2 extraction was performed under the conditions of temperature range from 35 to 45 °C and pressure, 150–250 bar for 2.5 h with a constant flow rate of 22 g/min. Extraction yields of lipids increased with pressure and temperature. The digestive enzyme activities of protease, lipase and amylase of SC-CO2 treated krill residues were slightly decreased comparing to organic solvent, n-hexane and acetone treated residues. In SC-CO2 treated samples, all of the digestive enzymes showed slightly higher temperature stability. In the other hand the crude extracts of SC-CO2 and n-hexane treated krill samples showed almost same optimum pH and pH stability for each of the digestive enzymes. It was also found in SDS-PAGE that there are no significant differences in protein patterns of the crude extracts of untreated, SC-CO2, n-hexane and acetone treated krill indicating no denaturation of proteins.
Journal: Journal of Industrial and Engineering Chemistry - Volume 18, Issue 4, 25 July 2012, Pages 1314–1319