Plant mediated green synthesis and antibacterial activity of silver nanoparticles using Crataegus douglasii fruit extract

• Ag nanoparticles were synthesized using Crataegus douglasii fruit.
• The applied method is simple, low cost and eco-friendly.
• The particle size of 29.28 nm was achieved in 24 h interaction time.
• The Ag nanoparticles showed antibacterial activity against Gram positive and Gram negative bacteria.
• The Ag nanoparticles can be used in pharmaceutical industry.

In this study, silver nanoparticles were synthesized using the Crataegus douglasii fruit extract as a reducing agent. The reaction process was monitored by UV–vis spectroscopy. Further characterization was carried out using scanning electron microscopy (SEM). To optimize the biosynthesis of silver nanoparticles, the effect of process variables such as extract concentrations, mixing ratio of the reactants, time and pH were also investigated. The SEM images showed silver nanoparticles with 29.28 nm size and nearly spherical shape at 24 h interaction time. The antibacterial activity of the synthesized silver nanoparticles was confirmed against Staphylococcus aureus and Escherichia coli.