Amperometric study of hydrogen peroxide biosensor with butadiene rubber as immobilization matrix

A carbon paste electrode bound by butadiene rubber has been newly constructed and its electrochemical properties have been investigated to test the practicability of the enzyme electrode. The binder of carbon powder was butadiene rubber dissolved in toluene and ground cabbage tissue was embedded in the matrix as an enzyme source. The electrode, which showed a mechanical robustness after volatilization of solvent, displayed good catalytic power (detection limit = 2.5 × 10−5 M, S/N = 2) and electrochemically irreversible characteristics. Its symmetry factor and the exchange current density of the electrode used were 0.23 and 1.71 × 10−3 A cm−2, respectively.