کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
229645 465038 2015 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Biochemical studies on the Natamycin antibiotic produced by Streptomyces lydicus: Fermentation, extraction and biological activities
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی مهندسی شیمی (عمومی)
پیش نمایش صفحه اول مقاله
Biochemical studies on the Natamycin antibiotic produced by Streptomyces lydicus: Fermentation, extraction and biological activities
چکیده انگلیسی

Natamycin “polyene” antibiotic was isolated from the fermentation broth of a Streptomyces strain No. AZ-55. According to the morphological, cultural, physiological and biochemical characteristics, and 16S rDNA sequence analysis, strain AZ-55 was identified as Streptomyces lydicus. It is active in vitro against some microbial pathogens viz: Staphylococcus aureus, NCTC 7447; Bacillus subtilis, NCTC 1040; Bacillus pumilus, NCTC 8214 ; Micrococcus luteus, ATCC 9341; Escherichia coli, NCTC 10416; Klebsiella pneumonia, NCIMB 9111; Salmonella typhi and Pseudomonas aeruginosa, ATCC 10145; S. cerevisiae, ATCC 9763; Candida albicans, IMRU 3669; Aspergillus flavus, IMI 111023; Aspergillus niger, IMI 31276; Aspergillus fumigatus, ATCC 16424; Fusarium oxysporum; Alternaria alternata and Rhizoctonia solani. The active metabolite was extracted using chloroform (1:1, v/v) at pH 7.0. The separation of the active ingredient of the antifungal agent and its purification were performed using both thin layer chromatography (TLC) and column chromatography (CC) techniques. The physico-chemical characteristics of the purified antibiotic viz. color, melting point, solubility, elemental analysis (C, H, N, O and S) and spectroscopic characteristics (UV absorbance and IR, mass & NMR spectra) have been investigated. This analysis indicates a suggested empirical formula of C33H47NO13. The chemical structural analysis with spectroscopic characteristics confirmed that the compound produced by S. lydicus, AZ-55 is Natamycin “polyene” antibiotic.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Saudi Chemical Society - Volume 19, Issue 4, July 2015, Pages 360–371
نویسندگان
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