Step-By-Step Crop Biopsy in Birds

Obtaining tissue samples for histopathologic examination is an important part of avian medicine. Harvesting biopsy samples from the crop, although a relatively simple procedure, can be a useful diagnostic tool (e.g., diagnosing proventricular dilation disease). As with all surgical patients, the bird must be properly assessed before the procedure, thereby determining its ability to withstand the stresses associated with general anesthesia, collection of the biopsy samples, and recovery. Careful tissue handling and proper instrumentation are essential for optimum pathological evaluation of the samples. Generally, 2 to 3 tissue samples of the affected area of the crop are submitted for diagnostic purposes. When testing for proventricular dilation disease, at least 1 blood vessel should be included within the tissue specimen. Rarely are there complications noted when obtaining crop biopsy samples if the proper surgical protocol is followed.