کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2447391 1553975 2013 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Hypoosmotic swelling test in epididymal ram (Ovis aries) spermatozoa
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
Hypoosmotic swelling test in epididymal ram (Ovis aries) spermatozoa
چکیده انگلیسی

The present study intended to develop the hypoosmotic swelling (HOS) test and to determine an appropriate hypoosmotic solution (HS) for its use in epididymal spermatozoa from ram, thus allowing the evaluation of the membrane functional integrity. We also tested if a 15 min exposure time could generate the same results as longer incubation times (30, 45 and 60 min). Ten different hypoosmotic solutions (50, 75, 100, 125, 150, 175, 200, 250, 275, and 300 mOsm/kg) were prepared from sodium citrate and fructose diluted in bidistilled water and were employed at 37 °C. According to the percentage of reacted spermatozoa, 75–100 mOsm/kg solutions were the most sensitive (P<0.05, n=15). Also, the different exposure times did not show any significant effect (P>0.05, n=15); therefore allowing 15 min to be considered sufficient incubation time.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Livestock Science - Volume 157, Issues 2–3, November 2013, Pages 618–622
نویسندگان
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