کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2461627 | 1555050 | 2013 | 10 صفحه PDF | دانلود رایگان |

Bronchial-associated immune development is critically important to protect neonates from respiratory infections. Herein, bronchial-associated immune development in formula-fed and sow-reared pigs is described. Colostrum-fed newborn piglets were fed medicated sow milk replacer formula beginning at 48 h of life or remained with the sow. Blood and tissues were sampled at one-week (d7) and three-weeks (d21) of age. Lymphocyte subpopulations, including T helper 2, cytotoxic T, memory T, and NK cells, in peripheral blood, mediastinal lymph nodes, and thoracic lymph nodes were identified using flow cytometry. Additionally, IL-1β, IL-6, IL-12, TNFα, TGF-β1, TGF-β2, IFNα, IFNβ, and dectin gene expression were analyzed by quantitative RT-PCR. Total IgG, IgM, and IgA concentrations in serum were analyzed. Dietary and developmental effects were observed. This set of baseline measurements provides a framework for future respiratory challenge studies where the effects of diet on the neonate's ability to resist and/or recover from infection can be tested.
► The effect of early nutrition (mothers milk vs. formula) and development (d7 vs. d21) on lymphocyte populations and gene expression was studied in piglets.
► Developmental effects were observed in lymphocyte populations in blood, mediastinal and thoracic lymph nodes.
► Lymphocyte populations in mediastinal and thoracic lymph nodes were also affected by diet.
► Developmental and dietary effects were observed in cytokine expression in lung, mediastinal and thoracic lymph nodes.
Journal: Veterinary Immunology and Immunopathology - Volume 151, Issues 1–2, 15 January 2013, Pages 63–72