کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2462168 1555066 2011 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Cloning and antibody recognition analysis of the canine 5-lipoxygenase gene
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
Cloning and antibody recognition analysis of the canine 5-lipoxygenase gene
چکیده انگلیسی

5-Lipoxygenase cDNA was prepared from canine white blood cells revealing the full-length message using an oligonucleotide capping method. The sequenced 5-Lipoxygenase open reading frame revealed a 2031 base pair message encoding a 676 amino acid protein. The amino acid sequence showed mild variation with the presumed canine sequence, as well as differences in important residues of known phosphorylation observed in other species. The sequence had between 86 and 92% homology with other species, revealing a highly conserved sequence. Confirmation of gene product identity was achieved through transient transfection of the gene in a V5-Histidine tagged pcDNA 3.1 vector into a known canine cell line. Both V5 antibody and 5-lipoxygenase antibody confirmed the gene product using Western blotting and immunoflourescence.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Veterinary Immunology and Immunopathology - Volume 142, Issues 3–4, 15 August 2011, Pages 276–279
نویسندگان
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