کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2462201 | 1555076 | 2010 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Expression, purification and monoclonal antibodies preparation of recombinant equine mature interleukin-18 Expression, purification and monoclonal antibodies preparation of recombinant equine mature interleukin-18](/preview/png/2462201.png)
IL-18 is a cytokine originally discovered as an important modulator of immune responses and subsequently shown to be pleiotropic. In this report, we expressed the recombinant equine mature interleukin-18 (rEMIL-18) in E. coli and purified it by nickel affinity gel column chromatography. Purified rEMIL-18 had biological activity commensurate with recombinant human IL-18, as determined by its synergistic effect with recombinant human IL-12 (rhIL-12) on the induction of IFN-γ gene expression in equine peripheral blood mononuclear cells (PBMC). Following intraperitoneal (i.p.) immunization of BALB/c mice with rEMIL-18, nine monoclonal antibodies (mAbs) against equine interleukin-18 (EIL-18) were obtained and characterized. These mAbs recognized different epitopes on equine mature interleukin-18 (EMIL-18) protein based on their reactivity with two peptides containing different amino acid sequences and one of these mAbs has neutralization activity against EIL-18 in an IFN-γ-induction assay.
Journal: Veterinary Immunology and Immunopathology - Volume 136, Issues 3–4, 15 August 2010, Pages 194–200