کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2463137 1555104 2008 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Polymorphism of Toll-like receptor 9 (TLR9) gene in sheep
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
Polymorphism of Toll-like receptor 9 (TLR9) gene in sheep
چکیده انگلیسی

Bacterial and synthetic DNA containing unmethylated CpG dinucleotides in particular sequence contexts, activates the vertebrate immune system through Toll-like receptor 9 (TLR9). In this study, we use PCR-single-strand conformational polymorphism (PCR-SSCP) analysis to investigate genetic variation in a key region of the ovine TLR9 gene. Three novel SSCP patterns, representing three different sequences, were identified. Either one or two different sequences were detected in individual sheep and all the sequences identified shared high homology to the TLR9 sequences from a variety of species, suggesting that these sequences represent allelic variants of the ovine TLR9 gene. Four single nucleotide polymorphisms (SNPs) were detected in the region amplified and two of them were non-synonymous substitutions that would result in amino acid changes. Variation detected here might have an impact on the structure and/or function of TLR9 and hence affect the immune response to pathogens.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Veterinary Immunology and Immunopathology - Volume 121, Issues 1–2, 15 January 2008, Pages 140–143
نویسندگان
, ,