کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2463161 1555112 2007 13 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Modulation of innate and acquired immunity by an estrogenic dose of genistein in gonadectomized cats
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
Modulation of innate and acquired immunity by an estrogenic dose of genistein in gonadectomized cats
چکیده انگلیسی

Dietary genistein has potential as a surrogate estrogen to restore normal control of food intake in cats following gonadectomy. However, since genistein affects immunological responses in other species it is important to determine if there is any immunological effect of genistein in cats, before long-term feeding of it could be considered. It was hypothesized that the minimum estrogenic dose of genistein (peak serum concentration 0.276 ± 0.055 μg/mL) would be associated with altered innate and acquired immunity in cats. Cats (n = 8 per group) were surgically neutered then treated daily with either 0.5 μg estradiol subcutaneously, 100 mg/kg genistein orally, or vehicle only for 38 days. Circulating CD4+ and CD8+ T cells, and CD21+ B cells were quantified using flow cytometry. Concanavalin-A induced lymphocyte proliferation was assayed using incorporation of tritiated thymidine. Indirect ELISA was used to assay serum IgG in response to a commercial vaccine. Neutrophil respiratory burst in response to opsonized zymosan was evaluated using flow cytometric detection of oxidized dihydrorhodamine. Delayed-type hypersensitivity was evaluated with formalin-killed Yersinia pseudotuberculosis. Genistein treatment decreased circulating CD8+ cells (P = 0.006), increased neutrophil respiratory burst (P = 0.034), and increased wheal formation at 24 h (P = 0.038) but decreased wheal formation at 72 h (P = 0.012) following intradermal challenge with killed Y. pseudotuberculosis. There were no significant differences between genistein treated cats and the other treatment groups in any other parameters. It is concluded that the minimum estrogenic dose of genistein alters selected leukocyte responses in cats. Unexpected effects of genistein suggest that extrapolation from one species to other species may not be appropriate in regards to the effects of genistein on immunity.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Veterinary Immunology and Immunopathology - Volume 117, Issues 1–2, 15 May 2007, Pages 42–54
نویسندگان
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