کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2463183 1555107 2007 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The effects of gene gun delivered pIL-3 adjuvant on skin pathology and cytokine expression
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
The effects of gene gun delivered pIL-3 adjuvant on skin pathology and cytokine expression
چکیده انگلیسی

The aim of this study was to investigate skin immunopathology following gene gun delivery of plasmid-encoding interleukin 3 (pIL-3) and hence explore the possible mechanisms of its adjuvant activity. Using the sheep as the experimental model, expressible pIL-3 was administered to the epidermis and the dermal/epidermal junction and its effects on the skin were assessed by histopathology, immunohistology and quantitative RT-PCR for a range of pro-inflammatory and immune response polarizing cytokines. Delivery of both functional and non-functional plasmids caused an acute inflammatory response with the infiltration of neutrophils and micro-abscess formation; however, the response to pIL-3 was more severe and was also associated with an early (24 h) infiltration of B cells and a later accumulation of CD172a−/CD45RA+ dendritic cells (DC).In terms of cytokine transcript expression, an early TNFα response was stimulated by gene gun delivery of plasmid-associated gold beads, which coincided with an immediate infiltration of neutrophils. However, only pIL-3 triggered the short-lived expression of IL-3 (peaking at 6 h) and significant long-term increases in both TNFα and IL-1β. pIL-3 did not affect the expression of the immune response polarizing cytokines, IL-10 and IL-12.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Veterinary Immunology and Immunopathology - Volume 119, Issues 3–4, 15 October 2007, Pages 233–242
نویسندگان
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