Functional development of immune response in rabbits

The aim of our study was to extend knowledge concerning postnatal development of the immune system in rabbits from two aspects. Firstly, capability of lymphocytes from peripheral blood, spleen, mesenteric, and popliteal lymph nodes to respond to Concanavalin A stimulation was investigated. Secondly, changes in the ability to produce antibodies against tetanus toxoid by rabbits during maturation were studied. Proliferation of lymphocytes was reduced in mesenteric lymph nodes in newborns, in PB up to the age of two weeks, and in popliteal lymph nodes up to the age of four weeks when compared to adults. High spontaneous lymphocyte proliferation that lasted up to the age of two weeks was recorded in spleen. The study of antibody response showed that even one-day-old rabbits were able to form specific antibodies of isotype IgM and IgG. Nevertheless, significantly lower formation of both isotypes was noted in one-day and two-week-old rabbits, and commencement of IgG isotype formation was delayed in one-day, two-week, and four-week-old rabbits when compared to adults.