کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2463437 1555121 2006 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Foals are interferon gamma-deficient at birth
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
Foals are interferon gamma-deficient at birth
چکیده انگلیسی

The increased vulnerability of foals to specific pathogens such as Rhodococcus equi is believed to reflect an innate immunodeficiency, the nature of which remains poorly understood. Previous studies have demonstrated that neonates of many species fail to mount potent Th1 responses. The current research investigates the ability of circulating and pulmonary lymphocytes of developing foals to produce interferon gamma (IFNγ). Peripheral blood mononuclear cells (PBMC) were prepared from up to 10 horse foals at regular intervals throughout the first 6 months of life. Bronchoalveolar lavage (BAL) samples were collected at 1, 3 or 6 months of age from three groups of five foals. The PBMC and BAL cells were stimulated in vitro and IFNγ production was measured by intracellular staining. In addition, RNA was extracted from freshly isolated and in vitro stimulated PBMC and BAL cells for quantitation of IFNγ gene expression by real time PCR. Newborn foals exhibited a marked inability to express the IFNγ gene and produce IFNγ protein. This deficiency was observed in both circulating and pulmonary lymphocytes. However, IFNγ gene expression and protein production increased steadily throughout the first 6 months of life, reaching adult levels within the first year of life. These findings suggest that foals are born with an inherent inability to mount a Th1-based cell mediated immune response which may contribute to their susceptibility to intracellular pathogens.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Veterinary Immunology and Immunopathology - Volume 112, Issues 3–4, 15 August 2006, Pages 199–209
نویسندگان
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