Efficacy of the paramunity inducer PIND-ORF in the treatment of canine parvovirus infection

• The aim of this study was to evaluate the efficacy of the paramunity inducer PIND-ORF in dogs with canine parvovirus (CPV) infection in a prospective, placebo-controlled, double-blinded trial, including 38 dogs.
• There was no significant difference in time to recovery, clinical signs, blood parameters, duration of virus shedding, and antibody titres between the placebo and PIND-ORF-treated dogs.
• The only significant difference was an increase in lymphocyte counts and antibody titres observed in the PIND-ORF group only.
• No significant effect of the paramunity inducer PIND-ORF on recovery and outcome could be demonstrated.

Canine parvovirus (CPV) infection is a common and severe disease particularly affecting young dogs. The paramunity inducer PIND-ORF is reported to stimulate the innate immune system and, if used as a supplementary medication, might lead to a more rapid improvement in clinical signs in dogs with CPV infection. The aim of this study was to evaluate the efficacy of PIND-ORF in dogs with CPV infection in a prospective, placebo-controlled, double-blinded trial using 38 dogs randomly assigned to two groups. Inclusion criteria were clinical signs consistent with CPV infection and a positive faecal CPV PCR. Dogs received either PIND-ORF (n = 20) or placebo (n = 18) and additional symptomatic treatment. Time to recovery and mortality rate were compared between the two groups. Clinical signs, complete blood counts (CBC), and serum protein and albumin concentrations were evaluated daily during hospitalisation and on day 14. Viral shedding and antibody titres were measured by faecal CPV PCR and serum neutralisation assay.There was no significant difference in time to recovery, clinical signs, blood parameters, duration of virus shedding, and antibody titres between the two groups. The only significant difference was an increase in lymphocyte counts and antibody titres observed in the PIND-ORF group only. Three dogs receiving placebo did not survive, but the mortality rate was not significantly different between groups (P = 0.097). No significant effect of PIND-ORF on recovery and outcome could be demonstrated.