کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2464187 1111782 2013 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Review of diagnostic plaque reduction neutralization tests for flavivirus infection
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
Review of diagnostic plaque reduction neutralization tests for flavivirus infection
چکیده انگلیسی

Flavivirus infections (including Japanese encephalitis, West Nile encephalitis and dengue fever/severe dengue) present a worldwide public health problem. Recent climate change may affect the geographical distribution of the arthropod vectors for these viruses and so the risk of flavivirus epidemics may increase. Many methods have been developed for the serological diagnosis of flavivirus infections, such as haemagglutination inhibition assay, enzyme-linked immunosorbent assay, and immunofluorescence in staining. However, the specificity of these assays varies.The plaque reduction neutralizing test (PRNT) using live viruses is currently the ‘gold standard’ for the differential serodiagnosis of flaviviruses. The specificity of results obtained with PRNT is better than that for other protocols and many laboratories apply the PRNT protocol to the differential serodiagnosis of flaviviruses. Here, recent refinements to the PRNT protocols with genetically modified recombinant viruses or reporter-harbouring virus-like particles are reviewed. Further, the problems associated with the differential serodiagnosis of flaviviruses using PRNT are discussed.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The Veterinary Journal - Volume 195, Issue 1, January 2013, Pages 33–40
نویسندگان
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