کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2464793 1111808 2011 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Development of a loop-mediated isothermal amplification assay for the detection of Mycobacterium bovis
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
Development of a loop-mediated isothermal amplification assay for the detection of Mycobacterium bovis
چکیده انگلیسی

Bovine tuberculosis caused by Mycobacterium bovis is an important zoonosis. In this study, a simple, rapid method for detecting this organism was developed based on loop-mediated isothermal amplification of the mpt83 gene. The technique will be of value in the clinical and field-based diagnosis of M. bovis and can differentiate it from other bacteria such as Corynebacterium diphtheriae, Streptococcus pneumoniae, β-haemolytic streptococci, Pseudomonas aeruginosa, Yersinia pseudotuberculosis, Staphylococcus aureus, and Klebsiella pneumoniae. The limit of detection was 10 copies/μL and the results were corroborated by PCR. The method was highly specific and more sensitive than PCR.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The Veterinary Journal - Volume 187, Issue 3, March 2011, Pages 393–396
نویسندگان
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