کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2464852 1111810 2010 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Binding sites of muscarinic and adrenergic receptors in gastrointestinal tissues of dairy cows suffering from left displacement of the abomasum
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
Binding sites of muscarinic and adrenergic receptors in gastrointestinal tissues of dairy cows suffering from left displacement of the abomasum
چکیده انگلیسی

Muscarinic acetylcholine (M) and adrenergic (AR) receptors mediate gastrointestinal motility. Using radioligand binding assays and real-time polymerase chain reaction, the densities of binding sites and mRNA levels of M2, M3, α2AD- and β2-AR were compared in muscle tissues from the abomasal fundus, pylorus, duodenum, caecum, and external loop of the spiral colon of eight cows with left displacement of abomasum (LDA), and of eight healthy cows. Specific binding of the [3H]-ligands to each of the four receptors was competitive and saturable. Binding sites of M2 (all intestinal sites), M3 (duodenum and caecum), and of α2AD-AR (abomasal fundus) were lower (P < 0.05) in cows with LDA than in healthy cows. The coefficients of correlation between binding sites and mRNA transcripts of receptors were dissimilar in cows with LDA and healthy cows. The decrease in densities of M (intestine) and of α2AD-AR (abomasum) receptors suggests their implication in the impairment of motility associated with or leading to LDA.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The Veterinary Journal - Volume 186, Issue 3, December 2010, Pages 328–337
نویسندگان
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