کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2465290 1111824 2010 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Early events in the pathogenesis of foot-and-mouth disease in cattle after controlled aerosol exposure
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
Early events in the pathogenesis of foot-and-mouth disease in cattle after controlled aerosol exposure
چکیده انگلیسی

The goal of this study was to identify the primary sites of replication of foot-and-mouth disease virus (FMDV) in cattle subsequent to aerogenous inoculation. A novel aerosol inoculation method was developed to simulate natural, airborne transmission and thereby allow the identification of early replication sites. Virus distribution after aerosol inoculation was compared at 24 h post inoculation with simple nasal instillation. Aerosol inoculation of FMDV consistently resulted in virus detection by real-time reverse transcriptase-polymerase chain reaction and viral isolation in the soft palate, pharynx, and lungs. Viral antigen was also detected in each of these tissues by immunohistochemistry. Aerosol exposure resulted in typical clinical signs of FMD when animals were kept alive long enough to develop disease. This aerosol infection method is highly reproducible regarding inoculum dose and volume, and allowed the detailed study of early events in FMDV-infected cattle. Extensive postmortem sampling and trimodal virus detection system allows a more precise determination of FMDV localization than previously reported.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The Veterinary Journal - Volume 183, Issue 1, January 2010, Pages 46–53
نویسندگان
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