کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2465426 1111829 2008 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Analytical validation of commercial immunoassays for the measurement of cardiovascular peptides in the dog
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
Analytical validation of commercial immunoassays for the measurement of cardiovascular peptides in the dog
چکیده انگلیسی

Immunoassays for the measurement of concentrations of the cardiovascular peptides pro-atrial natriuretic peptide (proANP), brain natriuretic peptide (BNPPen and BNPPhoe), endothelin-1 (ET-1Bio, ET-1IBL and ET-1Phoe) and big endothelin-1 (Big-ETBio and Big-ETIBL) were validated in canine serum by determination of intra-assay variability and dilutional parallelism. Commercial kits that showed good results were further validated by determination of intra- and inter-assay variability, dilutional parallelism and spiking recovery. Assays for proANP, BNPPhoe, ET-1IBL and Big-ETIBL showed acceptable results in the preliminary validation and were fully validated. The intra- and inter-assay variability was acceptable for all four assays, linearity was demonstrated and recovery rates were acceptable. The performances of the different immunoassays varied considerably, underscoring the importance of validation. Of the assays studied, proANP, BNPPhoe, ET-1IBL and Big-ETIBL produced precise, reproducible and accurate results and can be recommended for clinical application.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The Veterinary Journal - Volume 178, Issue 1, October 2008, Pages 85–90
نویسندگان
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