کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2465736 1111838 2009 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Cytokine mRNA responses in bovine epithelia during foot-and-mouth disease virus infection
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
Cytokine mRNA responses in bovine epithelia during foot-and-mouth disease virus infection
چکیده انگلیسی

Foot-and-mouth disease (FMD) remains the single most important constraint to international trade in live animals and animal products. The factors which regulate the pathogenesis and persistence of foot-and-mouth disease virus (FMDV) are poorly understood. mRNA levels of the inflammatory cytokines interleukin (IL)-1α, tumour necrosis factor (TNF)-α and the antiviral cytokines interferon (IFN)-α, β and γ in microdissected epithelium from cattle acutely infected with FMDV O UKG 34/2001 were quantified using laser microdissection in combination with a quantitative reverse transcription polymerase chain reaction assay. Cytokine mRNA responses in microdissected epithelia from the bovine tongue, coronary band and dorsal soft palate during the acute stage of FMDV infection were different. Significantly increased expression of cytokine mRNA was found in microdissected epithelia from the coronary band and tongue of FMDV-infected cattle and the extent of cytokine mRNA induction correlated with viral RNA loads. The results suggest that epithelia from different sites of an infected animal may mount different defences following infection and this may contribute to differences in their relative capacities to clear the virus.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The Veterinary Journal - Volume 179, Issue 1, January 2009, Pages 85–91
نویسندگان
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