کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2466134 | 1111852 | 2006 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Characterisation of transthyretin and retinol-binding protein in plasma and cerebrospinal fluid of dogs Characterisation of transthyretin and retinol-binding protein in plasma and cerebrospinal fluid of dogs](/preview/png/2466134.png)
The aim of this study was to investigate differences in concentrations of vitamin A, transthyretin (TTR) and retinol-binding protein (RBP) between plasma and cerebrospinal fluid (CSF) in dogs. RBP was detected using ELISA, and both RBP and TTR by Western blot analysis after separation on SDS–PAGE. Vitamin A was determined by high performance liquid chromatography.RBP and TTR as well as vitamin A were detected in all samples but at substantially lower concentrations in CSF compared to plasma. RBP in dog plasma showed a similar molecular mass to that of humans, whereas canine TTR had a lower molecular mass. Comparison between plasma and CSF showed that both RBP and TTR were of lower molecular mass in CSF. In CSF, RBP and retinol were present at 10–100-fold lower concentrations compared to plasma. Retinyl esters were present only in minute amounts in 5/17 samples. In conclusion, the CSF of dogs compared to humans is significantly different in terms of both quality and quantity of transport proteins for vitamin A.
Journal: The Veterinary Journal - Volume 171, Issue 3, May 2006, Pages 451–455