کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2495604 | 1116072 | 2012 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Ion-pair spectrophotometric estimation of gemifloxacin
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
پزشکی مولکولی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Ion-pair spectrophotometric estimation of gemifloxacin Ion-pair spectrophotometric estimation of gemifloxacin](/preview/png/2495604.png)
چکیده انگلیسی
Introduction: The main objective was to develop and validate a simple, accurate, precise, and sensitive ion-pair spectrophotometric extraction method for the assay of gemifloxacin mesylate (GFX) in pure, tablets and spiked human urine. Materials and Methods: The method is based upon the reaction of gemifloxacin with methyl orange, forming a yellow-colored complex in acidic medium, which is extracted in chloroform and analyzed. The extracted complexes showed absorbance maxima (λmax) found to be at 427 nm. Results: Beer's law was obeyed for a wide concentration range, i.e., 10-80 μg/mL as the extracted species seemed well defined and stable. The surface or an interphase adsorption phenomenon was not a problem. Optimization of the reaction was carried out with factors such as buffer strength, stability of complex, and molar ratio of drug: Dye and extraction time. The proposed method was validated as per the ICH guidelines. The recovery studies confirmed the accuracy and precision of the method. Conclusion: The above-mentioned method was a rapid tool for routine analysis of GFX in the bulk and pharmaceutical dosage forms.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Pharmaceutical Methods - Volume 3, Issue 1, JanuaryâJune 2012, Pages 26-30
Journal: Pharmaceutical Methods - Volume 3, Issue 1, JanuaryâJune 2012, Pages 26-30
نویسندگان
Satyabrata Sahu, Saroj Kumar Patro, Una Laxmi Narayan, Bamakanta Garnaik,