کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2495770 | 1116085 | 2012 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
In vivo and in vitro anti-inflammatory activity of Harrisonia perforata root extract
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
پزشکی مولکولی
پیش نمایش صفحه اول مقاله
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چکیده انگلیسی
Introduction:Harrisonia perforata root is one of the five medicinal plants in Bencha-Loga-Wichien remedy used for the treatment of fever in Thai traditional medicine. This study was aimed to investigate the anti-inflammatory effect and the action mechanism of the ethnolic extract from the root of H. perforatain vivo and in vitro. Methods: Male Wistar rats were divided into 6 groups and treated with either the ethanolic extract of H. perforata at dose 5-400 mg/kg or indomethacin 5 mg/kg or vehicle. Rat paw volume was measured at 1, 2, 3, 4, 5 and 6 h after carrageenan injection. The in vitro mechanism of the antiinfammatory response was investigated through the measurement of mRNA expression of proinflammatory cytokines, TNF-α, IL-6 and IL-1β in the macrophage cell. Lipopolysaccharide-stimulated macrophage, J774A.1 cell, were exposed to different concentration of Harrisonia perforata root extract (12.5-50 μg/ml). mRNA expression, TNF-α, IL-1β and IL-6, were determined by real-time RT-PCR. Results:H. perforata root extract 50-400 mg/kg significantly reduced the effect of acute inflammation in rat paw edema by 28.49%-65.05% at 2 hour after carrageenan injection. Cell viability were not significantly altered by the presence of H. perforata extract 3.125-50 μg/ml. It was found that the maximum inhibitory effect for TNF-α, IL-1β and IL-6 were 49.83, 47.27 and 32.16% respectively. The concentration of the extract produced maximum effect were 50 μg/ml for both TNF-α and IL-1β but for IL-6, it was 12.5 μg/ml. Conclusion: The ethanolic extract from H. perforata root possesses anti-inflammatory effect. This may occur via suppression of proinflammatory cytokines.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Pharmacognosy Journal - Volume 4, Issue 32, NovemberâDecember 2012, Pages 38-44
Journal: Pharmacognosy Journal - Volume 4, Issue 32, NovemberâDecember 2012, Pages 38-44
نویسندگان
Pattama Somsil, Nijsiri Ruangrungsi, Wacharee Limpanasitikul, Chandhanee Itthipanichpong,