Chemical constituents of Broussonetia luzonicus

The leaves of Broussonetia luzonicus afforded epitaraxerol (1), lupenone (2), squalene (3), β-carotene (4), vitamin K (5) and β-sitosterol (6), while the flowers yielded 2, 6, lupeol (7), betulin aldehyde fatty acid ester (8) and lupeol fatty acid ester (9). The compounds were isolated by silica gel chromatography and identified by NMR spectroscopy. Triterpenes 1, 2 and 8 were tested for cytotoxicity using the MTT assay. They did not exhibit cytotoxic effect against a human cancer cell line colon carcinoma (HCT 116). Results of the antimicrobial tests on 1, 2 and 8 indicated moderate antifungal activity against C. albicans and low antimicrobial activity against T. mentagrophytes, A. niger, S. aureus, E. coli, P. aeruginosa, and B. subtilis.