کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2577743 1129960 2014 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Triterpenes from the Protium heptaphyllum resin – chemical composition and cytotoxicity
ترجمه فارسی عنوان
تریترپنس از پروتیم هپاتیفولوم رزین ترکیب شیمیایی و سمیت سلولی
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
چکیده انگلیسی

Protium heptaphyllum (Aubl) Marchand, Burseraceae, is popularly used as an analgesic and anti-inflammatory agent. However, the cellular mechanism of action remains unknown. This study aims to evaluate the chemical composition of P. heptaphyllum resin and cytotoxicity on a breast cancer cell line (MCF-7). The chemical composition of the resin was determined by Gas Chromatography coupled to a Mass Spectrometer. The cytotoxicity was evaluated using an MTT assay. Annexin V-FITC, caspase-3, Angiotensin Converting Enzyme activity and Tumor Necrosis Factor alpha (TNF- α) assays were performed to evaluate apoptosis and inflammatory events. The resin consisted of triterpenes, such as α- and β-amyrin. Cytotoxicity was only observed in fractions enriched with α- and β-amyrin. The resin and fractions elicited antiproliferative activity, increased activity of caspase-3 and ACE, and a decrease in the TNF-α level. Altogether, the resin and fractions enriched with α- and β-amyrin promoted cytotoxicity and apoptosis.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Revista Brasileira de Farmacognosia - Volume 24, Issue 4, July–August 2014, Pages 399–407
نویسندگان
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