کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2776082 1567932 2016 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Aptima HR-HPV testing from Diff-Quick-stained fine-needle aspiration smears of oropharyngeal squamous cell carcinoma
ترجمه فارسی عنوان
آزمون Aptima HR-HPV از اسمیرهای آسپیراسیون با سوزن ظریف تفاوت سریع آغشته از کارسینوم سلول اسکواموس اروفارینژال
کلمات کلیدی
HPV؛ آسپیراسیون با سوزن ظریف؛ اسمیر تفاوت سریع؛ اوروفارنکس؛ سرطان سلولی اسکواموس ؛ Aptima
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوشیمی بالینی
چکیده انگلیسی

IntroductionHuman papillomavirus (HPV)-related oropharyngeal squamous cell carcinoma (SCC) is a biologically unique form of carcinoma that is important to identify for prognosis and treatment. The objective of this study was to evaluate the performance of the Aptima HPV assay using Diff-Quick (DQ) stained smears from fine-needle aspiration (FNA) of HPV-related oropharyngeal SCC.Materials and methodsPatients with a diagnosis of head and neck SCC who also had FNA sample demonstrating metastatic disease were identified. Using a mounting media-based cell transfer technique, approximately 200 tumor cells were selected and harvested from DQ-stained aspirate smeared slides. The selected cells were tested for high risk HPV using the Aptima HPV assay, an in vitro nucleic acid amplification test for the qualitative detection of E6/E7 viral messenger RNA from high-risk types of HPV. These results were compared with the p16 immunohistochemical staining of the corresponding surgical pathology specimens.ResultsTwenty-eight of 32 (87.5%) FNAs of p16-positive oropharyngeal SCC were positive for high-risk HPV by the Aptima assay and 18 of 18 (100%) FNAs of p16-negative SCC were negative for high-risk HPV by the Aptima assay.ConclusionsDQ-stained FNA smears can be used by the Aptima HPV assay to accurately detect high-risk HPVs in oropharyngeal SCCs with a sensitivity of 87.5% and a specificity of 100%. This provides an alternative to p16 immunohistochemical staining of FNA cell block material, which may not be available on all specimens.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of the American Society of Cytopathology - Volume 5, Issue 4, July–August 2016, Pages 221–226
نویسندگان
, , , , , , ,