کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2777984 1568042 2009 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Ocular prostaglandin production and morphology in mice lacking a single isoform of cyclooxygenase
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوشیمی بالینی
پیش نمایش صفحه اول مقاله
Ocular prostaglandin production and morphology in mice lacking a single isoform of cyclooxygenase
چکیده انگلیسی

Prostaglandins have many important roles in ocular physiology and are used clinically for the treatment of glaucoma. The aim of this study was to analyse the contribution of each cyclooxygenase isoform to ocular prostaglandin production using isoform-specific knockout mice. Ex vivo PGE2, 6-keto-PGF1α, and TXB2 production was measured from whole eyes, corneal tissue, uveoscleral tissue, lens, retina and optic nerve using enzyme-linked immunosorbant assays. Ocular immunohistochemical and histological analysis was also conducted for each genotype. Levels of each of the prostaglandins measured were significantly decreased in the corneal tissue, uveoscleral tissue, lens, retina and optic nerve of COX-1−/− mice in comparison with wild-type mice. In contrast, COX-2−/− mice had similar levels of ocular prostaglandin production to wild-type mice. These results suggest that COX-1 is the principal isoform responsible for prostaglandin production in the mouse eye. The absence of COX-1 or COX-2 did not appear to effect ocular development in these mice.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Prostaglandins, Leukotrienes and Essential Fatty Acids - Volume 81, Issues 5–6, November–December 2009, Pages 401–409
نویسندگان
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