کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2788652 | 1154442 | 2015 | 10 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Mesenchymal stem cells reside in a vascular niche in the decidua basalis and are absent in remodelled spiral arterioles Mesenchymal stem cells reside in a vascular niche in the decidua basalis and are absent in remodelled spiral arterioles](/preview/png/2788652.png)
• DMSCs were isolated from the decidua basalis region attached to the placenta.
• The maternal origin of DMSCs was verified.
• DMSCs expressed MSC markers and differentiated into mesodermal lineages.
• DMSCs were localised to the vascular niche of non-transformed spiral arterioles of the decidua.
• In fully transformed spiral arterioles, DMSCs were absent from the vascular niche.
IntroductionMaternal decidua basalis tissue attached to the placenta following delivery is a source of decidual mesenchymal stem cells (DMSCs). The in vitro characteristics of DMSCs have been partly defined but their in vivo function(s) are poorly understood. The anatomic location, or niche, provides clues regarding potential in vivo function(s) of DMSCs, but the niche has not been described.MethodsCells were isolated from the decidua basalis and flow cytometric analyses showed the expected phenotypic profile for MSC cell surface markers. In vitro, the cells differentiated into adipocytes, osteocytes, and chondrocytes. DMSCs were then stained with antibodies by immunofluorescence detection.ResultsImmunocytochemistry revealed that DMSCs were positive for FZD-9, STRO-1, 3G5, and α-SMA as expected and lacked expression of vWF and Ck7. Fluorescence in situ hybridization analysis showed the cultured cells were of maternal origin. Immunofluorescence was carried out on placental bed biopsies using the FZD-9, STRO-1, 3G5, and α-SMA antibodies. DMSCs were located in the vascular niche in decidua basalis. Immunofluorescence with antibodies to FZD-9, Ck7 and vWF revealed DMSCs in the vascular niche surrounding intact non-transformed spiral arterioles but DMSCs were absent in fully transformed spiral arterioles.DiscussionSpiral arteriole remodelling is a critical feature of human pregnancy. The DMSC niche was investigated in fully transformed and non-transformed spiral arterioles. DMSCs have not been previously implicated in spiral arteriole remodelling. The absence of DMSCs around fully transformed spiral arterioles suggests they are a target for replacement or destruction by invading placental extravillous trophoblast cells, which carry out spiral arteriole remodelling.
Journal: Placenta - Volume 36, Issue 3, March 2015, Pages 312–321