کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2789117 1154471 2013 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The transfer of ethyl glucuronide across the dually perfused human placenta
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شناسی تکاملی
پیش نمایش صفحه اول مقاله
The transfer of ethyl glucuronide across the dually perfused human placenta
چکیده انگلیسی

IntroductionAlcohol consumption during pregnancy can lead to Fetal Alcohol Spectrum Disorder (FASD), and because maternal self-reports are often unreliable, biomarkers of alcohol use are sometimes necessary to accurately determine fetal risk. Ethyl glucuronide (EtG), a direct metabolite of ethanol, has been detected in the meconium of infants born to mothers who consumed excessive alcohol during pregnancy. It is still unknown whether EtG detected in meconium originated from maternal hepatic glucuronidation of ethanol followed by subsequent placental transfer. Therefore, the objective of this study was to determine if EtG crosses the human placenta.MethodsThe transfer of EtG was measured using the ex vivo dual perfusion of an isolated human placental lobule. EtG (1 μg/mL) was added to the maternal circulation and samples were taken throughout the 1 h pre-experimental and 3 h experimental phases for measurement of EtG and markers of placental viability.ResultsAfter 3 h, the fetal-to-maternal ratio was 0.29 ± 0.02 and net maternal-to-fetal transfer was still occurring. Triplicate averages of EtG concentrations in perfused placental lobules ranged from 140 to 414 ng/g tissue. Placental validation markers were within normal ranges for all perfusions.DiscussionThe data show that EtG crosses the human placenta and primarily represents maternal exposure to alcohol.ConclusionsThis information can help with the development of more thorough biomarker screens for alcohol use during pregnancy.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Placenta - Volume 34, Issue 4, April 2013, Pages 369–373
نویسندگان
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