کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2789903 1154533 2010 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Contribution of Potassium in Human Placental Steroidogenesis
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شناسی تکاملی
پیش نمایش صفحه اول مقاله
Contribution of Potassium in Human Placental Steroidogenesis
چکیده انگلیسی
The role of K+ on steroidogenesis in isolated mitochondria from the human placenta was explored. Cholesterol uptake and progesterone synthesis were stimulated by K+, and by the further addition of ATP. In the presence of glibenclamide or quinine (inhibitors of the K+ channel mito-KATP), the synthesis of progesterone was improved, indicating that K+ acts outside the mitochondria. Valinomycin, a K+-ionophore, inhibited mitochondrial steroidogenesis only in the absence of K+. The mitochondrial K+ channel in human placental mitochondria is formed by the subunit Kir 6.1 which was detected by Western blot with polyclonal antibodies. These results suggest that K+ contributes placental mitochondrial steroidogenesis facilitating cholesterol uptake and intermembrane translocation through a mechanism non-dependent of the transport of K+ inside the mitochondria.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Placenta - Volume 31, Issue 10, October 2010, Pages 860-866
نویسندگان
, , , , ,