Implantation in the Macaque: Expansion of the Implantation Site During the First Week of Implantation

Data accumulated over several years of investigating implantation in macaque monkeys have been used to estimate the rate of expansion of the initial implantation site, the increase in volume of the site, and the rate of arterial invasion by cytotrophoblast columns. In addition the expansion of the secondary implantation site has also been estimated. The primary implantation site expands from an average diameter of 0.268 mm on day 10 to 4.93 mm on day 16–17. It expands in thickness from 0.064 mm on day 10 to 0.96 mm on day 16–17, and in volume from 0.0036 mm3 on day 10 to 18.34 mm3 on day 16–17. During this period of rapid expansion in extent and volume of the implantation site, trophoblast invades the endometrium, forms a lacunar stage, and initiates villus formation; consequently these very considerable changes in structure occur when the implantation is still very small yet growing rapidly. The secondary site expands from 0.23 mm in diameter on day 12 to 2.8 mm on day 17. The rate of penetration of cytotrophoblast into endometrial arteries diminishes from 0.602 mm per day on day 12 to 0.171 mm per day on day 16, using the straight-line method of estimation. This diminution in rate is consistent with the hypothesis that cytotrophoblast cells generated at the anchoring villi migrate over cytotrophoblast cells that have attached to the endothelium of the endometrial arteries in advancing the intraluminal columns of trophoblast. It is hoped that the summaries provided will be useful to investigators using macaque monkeys to analyze aspects of implantation in primates.