Female mating preference for own males on species and population level in Chromaphyosemion killifishes (Cyprinodontiformes, Nothobranchiidae)

Chromaphyosemion killifishes are a very promising taxon for the examination of general principles and mechanisms underlying speciation. The polygamous mating system and high degree of sexual dimorphism in this group suggest that sexual selection and female mating preferences play a crucial role in the current radiation process. Because the emergence of mating preferences for individuals of the own population during allopatry is a necessary precondition for speciation by sexual selection, either via diverging mate recognition traits or reinforcement, we examined whether females of different nominal species (Chromaphyosemion bivittatum vs. Chromaphyosemion volcanum) or populations (C. bivittatum “Toko” vs. C. bivittatum “Ilor”) prefer their own males as mating partners. For this purpose, two different methods were used. First, we conducted simultaneous choice tests where females could choose between two different males, and second, we counted the eggs produced by females in consecutive matings with different males. Both approaches produced the same result: females of the populations under examination preferred to mate with their own males. Preference for own males was symmetrical in all tests. Males, on the other hand, do not discriminate between females, not even on the level of nominal species. Thus, our results are in accordance with the hypothesis that female choice is an important factor for speciation in Chromaphyosemion. Additionally, we present some preliminary data on post-zygotic isolation between the populations.