کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2808506 1157764 2009 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
In vivo electrophysiological effects of insulin in the rat brain
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی علوم غدد
پیش نمایش صفحه اول مقاله
In vivo electrophysiological effects of insulin in the rat brain
چکیده انگلیسی

Brain insulin has widespread metabolic, neurotrophic, and neuromodulatory functions and is involved in the central regulation of food intake and body weight, learning and memory, neuronal development, neuronal apoptosis, and aging. To understand the neuromodulatory role of insulin, we aimed to characterize its yet undefined in vivo electrophysiological effects. We elected to record from the cerebellar cortex because this region has average insulin concentration and insulin receptor content in relation to the whole brain, and has been previously shown to be a target for insulin signaling. We used in vivo microiontophoresis to apply insulin juxtaneuronally while simultaneously recording changes in spontaneous neuronal activity. The analysis included 553 significant neuronal responses to insulin and other related agents recorded from 47 cerebellar neurons of the rat. We found that (1) insulin stimulation produced instant and reversible electrophysiological effects on all of the recorded neurons, and that (2) these effects were mostly dependent on prior or simultaneous GABA application (94–96%). Specifically, (a) inhibitory responses to insulin were the most common (58–62%), and were dose-dependent with respect to GABA pretreatments and blocked by co-administration of the insulin receptor inhibitor HNMPA. (b) In the second largest set of neurons (32–38%) insulin decreased the magnitude of GABA inhibitions when co-applied. (c) In contrast, only a small number of neurons showed GABA-independent responses to insulin application (4–6%), which were exclusively neuronal excitations. The present findings demonstrate that insulin has direct electrophysiological effects on central neurons in vivo and these effects are highly influenced by GABA-ergic inputs.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuropeptides - Volume 43, Issue 4, August 2009, Pages 283–293
نویسندگان
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