Role of substance P (SP) and calcitonin gene-related peptide (CGRP) in gibbon-ape-leukemia virus (GALV) transduction of CD34+ cells

AimsOptimization of transduction condition is an important goal to improve gene transduction. Therefore, we aimed to assess the effect of SP and/or CGRP as novel growth/transducing factors on the efficacy of CD34+ transduction.MethodsCD34+ cells were transduced with Gibbon-Ape-Leukemia virus (GALV), containing Neomycin gene. CD34+ cells were transduced with GALV presence of SP and/or CGRP. Real Time PCR and colony formation assay (CFU-C) was performed.ResultsViral vectors titration on Hela cells indicated transduction efficiency of 1 × 106 CFU/ml. Real Time PCR of Neo and CFU-C showed stimulatory role of SP on gene transfer 5.9% and 14.84% compared to 3.6% and 12.58% in control group, while opposite role observed for CGRP 0.89% and 7.86%. Both SP and CGRP showed no significant effect in these assays.DiscussionThis study showed including of SP in growth factor cocktail is beneficial for CD34+ transduction, which could be applied to genetic modification procedures.