کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2808609 1569074 2008 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Central administration of angiotensin-(1–7) stimulates nitric oxide release and upregulates the endothelial nitric oxide synthase expression following focal cerebral ischemia/reperfusion in rats
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی علوم غدد
پیش نمایش صفحه اول مقاله
Central administration of angiotensin-(1–7) stimulates nitric oxide release and upregulates the endothelial nitric oxide synthase expression following focal cerebral ischemia/reperfusion in rats
چکیده انگلیسی

Angiotensin-(1–7) [Ang-(1–7)] is an endogenous peptide of the renin–angiotensin system with several beneficial effects that are often opposite to those attributed to angiotensin II (Ang II). Since there are no data available so far on the role of Ang-(1–7) after cerebral ischemia/reperfusion, in this paper, we investigated the central administration of Ang-(1–7) modulates in vivo the nitric oxide(NO) release and the endothelial NO synthase (eNOS) expression following focal cerebral ischemia/reperfusion in rats. Cerebral ischemia–reperfusion injury was induced by intraluminal thread occlusion of middle cerebral artery in the adult male rats. The levels of NO in ischemic tissues were measured by NO detection kits. Reverse transcription (RT)-PCR and western blot were used to determine messenger RNA (mRNA) and protein levels of the eNOS in ischemic tissues. The cerebral ischemic lesion resulted in a significant increase of NO release at 3 and 6 h compared with sham operation group in our model after reperfusion, whereas both medium and high doses Ang-(1–7) markedly enhanced NO levels at 3–24 h, and 3–72 h after reperfusion, respectively. In addition, NO release increased was significantly induced by high-dose Ang-(1–7) compared with medium-dose Ang-(1–7) at 24–72 h after reperfusion. Medium and high-dose Ang-(1–7) significantly stimulated eNOS activation when compared with artificial cerebrospinal fluid (aCSF) treatment group at 3, 6, 12, 24, and 48 h after reperfusion, however, no significant changes in eNOS expression were found between medium and high-dose Ang-(1–7) at different times after the ischemic insult. These findings indicate that medium and high-dose Ang-(1–7) stimulate NO release and upregulate eNOS expression in ischemic tissues following focal cerebral ischemia/reperfusion in rats.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuropeptides - Volume 42, Issues 5–6, October–December 2008, Pages 593–600
نویسندگان
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