کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2814769 | 1159828 | 2016 | 8 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: MiR-455-5p acts as a novel tumor suppressor in gastric cancer by down-regulating RAB18 MiR-455-5p acts as a novel tumor suppressor in gastric cancer by down-regulating RAB18](/preview/png/2814769.png)
• MiR-455-5p is down-regulated in gastric cancer.
• MiR-455-5p can inhibit gastric cancer cell proliferation and invasion.
• RAB18 is the direct target of miR-455-5p.
• MiR-455-5p acts as a tumor suppressor in gastric cancer.
AimTo detect the potential regulation pathway of microRNA-455-5p (miR-455-5p) and RAB18, member RAS oncogene family (RAB18) in gastric cancer (GC) cells and tissues and discuss the clinical significance of miR-455-5p in GC genesis and progression.MethodsReal-time PCR was used to measure mRNA level of miR-455-5p in GC, TargetScan and dual luciferase assay were used to predict and demonstrate the candidate target gene of miR-455-5p, Western blot were utilized to detect the protein level of RAB18. Cell function assays were also performed to determine the function of miR-455-5p in GC.ResultsmiR-455-5p was reduced significantly in gastric cancer cells and tissues compared with the corresponding normal control, the lower expression of miR-455-5p was related to advanced clinical stage in gastric cancer, re-expression of miR-455-5p could inhibit human GC cell proliferation and invasion, overexpression of miR-455-5p could also promote GC cell apoptosis. Furthermore, we found that RAB18 was a candidate target of miR-455-5p. Re-expression of miR-455-5p could inhibit the protein level of RAB18.ConclusionMiR-455-5p might serve as a novel biomarker in gastric cancer by targeting RAB18.
Journal: Gene - Volume 592, Issue 2, 5 November 2016, Pages 308–315