کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2814788 | 1159830 | 2016 | 11 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: In silico analysis for prediction of degradative capacity of Pseudomonas putida SF1 In silico analysis for prediction of degradative capacity of Pseudomonas putida SF1](/preview/png/2814788.png)
Research highlights
• Study demonstrates broad and flexible degradative capacity of bacteria for its potential application in bioremediation.
• Approach used in the analysis discriminates functional proteins which otherwise shows strong DNA homology.
• Different genetic arrangement acquired in various genotypes brings out similar phenotypic expression under stress.
The study employs draft genome sequence data to explore p-nitrophenol (PNP) degradation activity of Pseudomonas putida strain SF-1 at a genomic scale. Annotation analysis proposes that the strain SF1 not only possesses the gene cluster for PNP utilization but also for the utilization of benzoate, catechol, hydroxybenzoate, protocatechuate, and homogentisate. Further, the analysis was carried out to understand more details of PNP 4-monooxygenase and its regulator. A comparative analysis of PNP 4-monooxygenase from SF1 was carried out for prediction of its tertiary structure; and also its binding affinity with PNP, FAD, NADH and NADPH using FlexX docking. The tertiary structure of regulator was also predicted along with its conserved DNA binding residues. Regulator binding site (RBS) and promoter region were mapped for the PNP degradation gene cluster. Based on genome sequence analysis, the study unveiled the genomic attributes for a versatile catabolic potential of Pseudomonas putida strain SF-1 for different aromatic compounds.
Journal: Gene - Volume 591, Issue 2, 15 October 2016, Pages 382–392